SubtiBank SubtiBank
gapB [2019-07-09 08:24:04]
You are currently viewing an outdated version of SubtiWiki. Please use the newest version!

gapB [2019-07-09 08:24:04]

glyceraldehyde-3-phosphate dehydrogenase, NADP-dependent, gluconeogenic enzyme, forms a transhydrogenation cycle with GapA for balancing of NADPH
Locus
BSU29020
Isoelectric point
6.45
Molecular weight
37.32 kDa
Protein length
340 aa Sequence Blast
Gene length
1023 bp Sequence Blast
Function
anabolic enzyme in gluconeogenesis
Product
glyceraldehyde-3-phosphate dehydrogenase 2
Essential
no
E.C.
1.2.1.59
Synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      
Loading

Categories containing this gene/protein

  • Metabolism → Carbon metabolism → Carbon core metabolism → Gluconeogenesis

    • Gene

    Coordinates
    2,967,032 → 2,968,054

    Phenotypes of a mutant

  • inactivation of gapB reduces sporulation efficiency to 0.3% that of wild type cells; delayed entry into sporulation and fewer sporulating cells PubMed

    • The protein

    Catalyzed reaction/ biological activity

  • D-glyceraldehyde 3-phosphate + phosphate + NAD(P)+ = 3-phospho-D-glyceroyl phosphate + NAD(P)H (according to Swiss-Prot)
  • This reaction is part of the gluconeogenesis

    • Protein family

  • glyceraldehyde-3-phosphate dehydrogenase family (according to Swiss-Prot)

    • Paralogous protein(s)

  • GapA

    • Kinetic information

  • Michaelis-Menten PubMed

    • Domains

  • Nucleotid bindinge domain (12-13)
  • 2x Glyceraldehyde 3-phosphate binding domain (151-153) & (210-211)

    • Cofactors

  • NADP+ (preferentially) and NAD+ PubMed

    • Structure

  • 3PRL (from B. halodurans)

    • Localization

  • Cytoplasm (Homogeneous) PubMed

    • Additional information

  • degraded after a shift from malate to glucose with an estimated half-life time of approx. 3 hours by ClpC-ClpP PubMed

    • Expression and Regulation

    Operons

    Genes
    gapB-speD
    Description
    PubMed

    Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon

    • Regulatory mechanism

  • CcpN: repression, PubMed, in CcpN regulon

    • Regulation

  • gapB: repressed in the presence of glucose (CcpN) PubMed

    • Additional information

  • 'speD': the mRNA is substantially stabilized upon depletion of RNase Y (the half-life of the monocistronic 'speD' mRNA increases from 1.4 to 36 min) PubMed
    • view in new tab

    Biological materials

    Mutant

  • MGNA-A121 (gapB::erm), available at the NBRP B. subtilis, Japan
  • GP701 (gapB::spec), available in Stülke lab
  • 1A1004 ( gapB::erm), [Pubmed| ], available at BGSC
  • BKE29020 (ΔgapB::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGTTGGACACCCCTTATC, downstream forward: _UP4_TAAAATAAGGTCATGGACAC
  • BKK29020 (ΔgapB::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGTTGGACACCCCTTATC, downstream forward: _UP4_TAAAATAAGGTCATGGACAC

    • Labs working on this gene/protein

  • Stephane Aymerich, Microbiology and Molecular Genetics, INRA Paris-Grignon, France

    • References

    Loading